Objective: To find an effective method of isolating, purifying, primary culturing and identifying the rat colonic smooth muscle cells. To investigate the effective mechanism of Chinese herb Lappula echinata Gilib ( LEG) on relaxation of smooth muscle cells. 目的:建立离体大鼠结肠平滑肌细胞分离、纯化、原代培养及鉴定的方法,在细胞水平研究东北鹤虱药物血清的平滑肌松弛作用机制。
Methods: Extracts and parts from Lappula echinata were screened by the degree of inhibiting the motility of smooth muscle firstly. 方法:以家兔离体回肠平滑肌自律性收缩抑制率为指标,对东北鹤虱的提取物进行药理活性指导性筛选。